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Answered by Dr.T.V.Rao MD Medical Microbiology needs a good thinking and execution of the knowledge it explores the various microbial life forms that comprise the sciences of bacteriology, mycology, virology and parasitology, and illustrates the impact microbes have on our lives by explaining how they function as distinct entities within a complex biosphere. The critical role of the microbiology laboratory in infectious disease diagnosis calls for a close, positive working relationship between the physician and the microbiologists who provide enormous value to the health care team Evaluating cause and effect relationships, such as microbial causes of disease, by the scientific method are good exercises for developing critical thinking skills. At an elementary level, the physician needs answers to 3 very basic questions from the laboratory: Is my patient’s illness caused by a microbe? If so, what is it? What is the susceptibility proļ¬le of the organism so therapy can be targeted? To meet those needs, the laboratory requires very different information
Let us start with Basic work we can start doing in our Laboratory
The practical use of Microscope is immense in making vital decisions in Bacteriology parasitology, and Mycology
THE GREAT STEP IN MICROBIOLOGY REAMIN WITH SCEINTIFIC USE OF A MICROSCOPE
Mastering the Gram’s staining and observation lead to mastering of Bacteriology and putting little more efforts in staining for Acid Fast staining further enhances our diagnostic capabilities to practical working,
Microscopy using the microscope - The setting up of a microscope is a basic skill of microbiology yet it is rarely mastered. Often entrusted to most junior staff and technicians, Only when it is done properly can the smaller end of the diversity of life be fully appreciated and its many uses in practical microbiology, from aiding identification to checking for contamination, be successfully accomplished. However when we look at CSF specimens in night hours, the utility is immense even if we find few bacteria, and helps in taking the vital decisions in time of crisis, The amount of magnification of which a microscope is capable is an important feature but it is the resolving power that determines the amount of detail that can be seen. The microscope is absolutely essential to the microbiology lab: most microorganisms cannot be seen without the aid of a microscope, save some fungi. And, of course, there are some microbes which cannot be seen even with a microscope, unless it is an electron microscope, such as the viruses
As soon as you see the specimen, STOP using the coarse adjustment, and switch over to the fine adjustment knob. After focusing at the beginning with the coarse adjustment knob, it is NOT TOUCHED AGAIN. All focusing will now be done with the fine adjustment knob.
Bacteria and yeast - Yeast can be seen in unstained wet mounts at magnifications ×100. Bacteria are much smaller and can be seen unstained at ×400 but only if the microscope is properly set up and all that is of interest is whether or not they are motile. A magnification of ×1,000 and the use of an oil immersion objective lens for observing stained preparations are necessary for seeing their characteristic shapes and arrangements. The information gained, along with descriptions of colonies, is the starting point for identification of genera and species, but further work involving physiology, biochemistry and molecular biology is then needed.
Moulds Routine identification of moulds is based entirely on the appearance of colonies to the naked eye and of the mycelium and spores in microscopical preparations. Mould mycelium and spores can be observed in unstained wet mounts at magnifications of ×100 although direct observations of ‘mouldy’ material through the lid of a Petri dish or specimen jar at lower magnifications with the plate microscope are also informative (but keep the lid on!). Routine identification of moulds is based entirely on the appearance of colonies to the naked eye and of the mycelium and spores in microscopical preparation helps support fungal etiology.
NEVER FORGET THAT THE MASTERING OF DIGANOSTIC MICROBIOLOGY STARTS WITH OPTIMAL USE OF MICROSCOPY
NEVER FORGET SIMPLE PATEINCE MAKE YOU A BETTER MICROBIOLOGIST
Ref – Basic use of Microscope in Diagnostic Microbiology WEB RESOURCES
Dr.T.V.Rao MD Professor of Microbiology
Answered by Dr.T.V.Rao MD Medical Microbiology needs a good thinking and execution of the knowledge it explores the various microbial life forms that comprise the sciences of bacteriology, mycology, virology and parasitology, and illustrates the impact microbes have on our lives by explaining how they function as distinct entities within a complex biosphere. The critical role of the microbiology laboratory in infectious disease diagnosis calls for a close, positive working relationship between the physician and the microbiologists who provide enormous value to the health care team Evaluating cause and effect relationships, such as microbial causes of disease, by the scientific method are good exercises for developing critical thinking skills. At an elementary level, the physician needs answers to 3 very basic questions from the laboratory: Is my patient’s illness caused by a microbe? If so, what is it? What is the susceptibility proļ¬le of the organism so therapy can be targeted? To meet those needs, the laboratory requires very different information
Let us start with Basic work we can start doing in our Laboratory
The practical use of Microscope is immense in making vital decisions in Bacteriology parasitology, and Mycology
THE GREAT STEP IN MICROBIOLOGY REAMIN WITH SCEINTIFIC USE OF A MICROSCOPE
Mastering the Gram’s staining and observation lead to mastering of Bacteriology and putting little more efforts in staining for Acid Fast staining further enhances our diagnostic capabilities to practical working,
Microscopy using the microscope - The setting up of a microscope is a basic skill of microbiology yet it is rarely mastered. Often entrusted to most junior staff and technicians, Only when it is done properly can the smaller end of the diversity of life be fully appreciated and its many uses in practical microbiology, from aiding identification to checking for contamination, be successfully accomplished. However when we look at CSF specimens in night hours, the utility is immense even if we find few bacteria, and helps in taking the vital decisions in time of crisis, The amount of magnification of which a microscope is capable is an important feature but it is the resolving power that determines the amount of detail that can be seen. The microscope is absolutely essential to the microbiology lab: most microorganisms cannot be seen without the aid of a microscope, save some fungi. And, of course, there are some microbes which cannot be seen even with a microscope, unless it is an electron microscope, such as the viruses
As soon as you see the specimen, STOP using the coarse adjustment, and switch over to the fine adjustment knob. After focusing at the beginning with the coarse adjustment knob, it is NOT TOUCHED AGAIN. All focusing will now be done with the fine adjustment knob.
Bacteria and yeast - Yeast can be seen in unstained wet mounts at magnifications ×100. Bacteria are much smaller and can be seen unstained at ×400 but only if the microscope is properly set up and all that is of interest is whether or not they are motile. A magnification of ×1,000 and the use of an oil immersion objective lens for observing stained preparations are necessary for seeing their characteristic shapes and arrangements. The information gained, along with descriptions of colonies, is the starting point for identification of genera and species, but further work involving physiology, biochemistry and molecular biology is then needed.
Moulds Routine identification of moulds is based entirely on the appearance of colonies to the naked eye and of the mycelium and spores in microscopical preparations. Mould mycelium and spores can be observed in unstained wet mounts at magnifications of ×100 although direct observations of ‘mouldy’ material through the lid of a Petri dish or specimen jar at lower magnifications with the plate microscope are also informative (but keep the lid on!). Routine identification of moulds is based entirely on the appearance of colonies to the naked eye and of the mycelium and spores in microscopical preparation helps support fungal etiology.
NEVER FORGET THAT THE MASTERING OF DIGANOSTIC MICROBIOLOGY STARTS WITH OPTIMAL USE OF MICROSCOPY
NEVER FORGET SIMPLE PATEINCE MAKE YOU A BETTER MICROBIOLOGIST
Ref – Basic use of Microscope in Diagnostic Microbiology WEB RESOURCES
Dr.T.V.Rao MD Professor of Microbiology
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